Ginkgo biloba L

Indications
For chest pain, heart pain, stroke, hemiplegia, tongue stiffness and slurred speech caused by blood stasis, stable angina pectoris of coronary heart disease, and cerebral infarction.
Pharmacological effects
Ginkgo biloba is still used in Chinese medicine to treat memory loss, stomach pain, dysentery, hypertension, mental stress and respiratory problems such as asthma, bronchitis and poor circulation and the anxiety caused by it. The active ingredients of ginkgo are terpenes, including ginkgolides and bilobalide. These ginkgo flavonoids-glycoside components have strong antioxidant and free radical scavenging abilities.
Drug analysis
Method name
Ginkgo biloba extract-determination of terpenoid lactones-HPLC
Scope of application
This method uses HPLC to determine the content of terpenoid lactones in ginkgo biloba extract.
This method is applicable to extracts made from processed ginkgo biloba.
Principle of the method
The product is extracted with ethyl acetate, the extracts are combined and the solvent is recovered until dry, the residue is dissolved with methanol, shaken and filtered, and the filtrate is put into a high performance liquid chromatograph for chromatographic separation. The content is calculated by using an evaporative light scattering detector.

Reagents
1. Methanol
2. n-Propanol
3. Tetrahydrofuran
4. 2% hydrochloric acid solution
5. Ethyl acetate
6. 5% sodium acetate solution
Instruments
1. Instruments
(1) High performance liquid chromatograph
(2) Chromatographic column

Application areas
1. Food and health care products industry
Ginkgo biloba flavonoids have antioxidant properties and can be added to oils and pastries as antioxidants. The total flavonoids are mostly yellow and have a wide solubility. They are both water-soluble and fat-soluble, so the total flavonoids can act as a colorant. Ginkgo biloba is processed into ultrafine powder and added to food. Ginkgo biloba is ultrafinely ground and added to pastries, biscuits, noodles, candies, and ice cream at 5% to 10% to make ginkgo biloba food with health benefits.
Ginkgo biloba extract is used as a food additive in Canada and has been approved as an over-the-counter drug in Germany and France. Ginkgo biloba is included in the United States Pharmacopoeia (24th edition). Ginkgo biloba extract can be used as a dietary supplement in the United States. There are more than 30 ginkgo products in the world, and the main dosage forms include: ginkgo leaf tablets, ginkgo oral liquid, ginkgo lactone injection, ginkgo biloba injection, ginkgo ketone ester pills, compound ginkgo leaf granules, ginkgo leaf extract, etc. From 2009 to 2011, the sales and DDDs ranking of ginkgo leaf preparations ranked the top three among the commonly used traditional Chinese medicines for cardiovascular diseases.
Ginkgo leaves contain a small amount of proanthocyanidins and urushiol acids, which are still toxic to the human body. When ginkgo leaves are used as raw materials for food processing, they must be specially treated to reduce the content of proanthocyanidins and urushiol acids. However, within the currently used dosage range, there is no acute and chronic toxicity and no teratogenic effect. The Ministry of Health approved ginkgo leaf extract as a new food additive in 1992. In recent years, total flavonoids from ginkgo leaves have been widely used in the food industry, and the research and development of ginkgo leaves has broad prospects.
2. Daily chemical industry
The combination of ginkgo leaf extract and skin absorption accelerator can also prevent the irritation caused by the adhesive used in external ointments and patches, which can be emulsions, ointments, creams, sols and plasters. The skin care products containing GBE on the market mainly include anti-aging cleansers, shower gels, lipsticks, eye creams, etc. GBE can also be used in the production of hair growth and hair care products. Chen Yan and others even added ginkgo leaf extract into toothpaste, which, according to their research, has a certain anti-caries effect.
Octadecylsilane bonded silica gel is used as the filler, and the theoretical plate number calculated based on the bilobalide peak should be no less than 2500.
(3) Evaporative light scattering detector
2. Chromatographic conditions
(1) Mobile phase: n-Propanol: tetrahydrofuran: water = 1:15:84
(2) Column temperature: room temperature
Sample preparation
Weigh the sample
Accurately weigh 0.15g of this product as the sample.
Preparation of reference solution
Accurately weigh appropriate amounts of bilobalide reference, ginkgolide A reference, ginkgolide B reference and ginkgolide C reference, and add methanol to prepare mixed solutions containing 2 mg, 1 mg, 1 mg and 1 mg of each per mL. Alternatively, accurately weigh 0.15 g of ginkgo leaf reference extract labeled with bilobalide, ginkgolide A, ginkgolide B and ginkgolide C content, and prepare reference extract solution in the same way as the test solution.